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81.
C.B. Magnusson K.P. Poulsen J.A. Budde F.A. Hartmann N.A. Aulik S.M. Raabis A.S.D. Moreira B.J. Darien S.F. Peek 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):469-473
Background
Periodic lack of availability and high cost of commercially produced isotonic fluids for intravenous (IV) use in horses have increasingly led to use of home‐made or commercially compound fluids by veterinarians. Data regarding the quality control and safety of compounded fluids would be of benefit to equine veterinarians.Objectives
To compare electrolyte concentrations, sterility, and endotoxin contamination of commercially available fluids to 2 forms of compounded isotonic crystalloid fluids intended for IV use in horses.Methods
Prospective study. Two methods of preparing compounded crystalloids formulated to replicate commercial Plasma‐Lyte A (Abbott, Chicago, IL) were compared. One formulation was prepared by a hand‐mixed method involving chlorinated drinking water commonly employed by equine practitioners, and the other was prepared by means of ingredients obtained from a commercial compounding pharmacy. The variables for comparison were electrolyte concentrations, sterility, and presence of endotoxin contamination.Results
Electrolyte concentrations were consistent within each product but different between types of fluids (P < 0.0001). Hand‐mixed fluids had significantly more bacterial contamination compared to commercial Plasma‐Lyte A (P = 0.0014). One of the hand‐mixed fluid samples had detectable endotoxin contamination.Conclusions and Clinical Importance
Chlorinated drinking water is not an acceptable source of water to compound isotonic fluids for IV administration. Equine practitioners should be aware of this risk and obtain the informed consent of their clients. 相似文献82.
Matheus V. L. Moreira Maria C. de Andrade Gustavo O. Fulgêncio Ingeborg M. Langohr Roselene Ecco 《Veterinary ophthalmology》2018,21(5):535-538
An indoor‐only, 5‐year‐old, spayed female domestic shorthair cat presented for an ophthalmic examination of the left eye. An intraocular tumor with secondary glaucoma and blindness was diagnosed; the globe was enucleated and sent for histopathological examination. Gross examination revealed a solid white mass filling the entire vitreous space and replacing the iris and ciliary body. The lens and retina appeared to be similarly replaced by the neoplasm. Histological examination revealed a complete loss of the internal ocular structures, with a ruptured capsule as the only remnant of the lens within an extensive malignant mesenchymal neoplastic cell proliferation. The cells were polygonal, with well‐defined cytoplasmic borders and abundant weakly basophilic cytoplasm, embedded within the islands of chondroid matrix. No neoplastic invasion of the sclera was apparent. The animal died 6 months after the enucleation due to respiratory distress. Gross examination revealed numerous firm, white to tan nodular masses with smooth to mildly irregular surfaces dispersed throughout the parietal pleura, thoracic surface of the diaphragm, tracheobronchial and mediastinal lymph nodes, pericardium, and lungs. On cross‐section, the neoplastic nodules were solid and variably translucent, resembling hyaline cartilage. Histologically, these nodules were similar to the neoplasm identified earlier in the left globe. Metastasis of post‐traumatic ocular chondrosarcoma has not yet been described in cats. This is therefore believed to be the first report of metastases of this type of neoplasm in cats. This case adds to the limited set of data on the outcome of this type of tumor. 相似文献
83.
Barros Lílian Moreira Prochnow Daiane de Oliveira Victoria Freitas da Silva Airton Rosa de Oliveira Antonio Costa da Maia Luciano Carlos 《Journal of Crop Science and Biotechnology》2019,22(1):31-36
Journal of Crop Science and Biotechnology - The morphological characterization allows one to estimate and evaluate the genetic distance among different varieties, identifying heterotrophic groups... 相似文献
84.
Andr Moreira Souza Rodrigo de Andrade Santos Weigert Elaine Parros Machado de Sousa Lucas Tassoni Andrietta Ricardo Vieira Ventura 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2022,139(1):100-112
The objective of our study was to provide practical directions on the storage of genomic information and novel phenotypes (treated here as unstructured data) using a non-relational database. The MongoDB technology was assessed for this purpose, enabling frequent data transactions involving numerous individuals under genetic evaluation. Our study investigated different genomic (Illumina Final Report, PLINK, 0125, FASTQ, and VCF formats) and phenotypic (including media files) information, using both real and simulated datasets. Advantages of our centralized database concept include the sublinear running time for queries after increasing the number of samples/markers exponentially, in addition to the comprehensive management of distinct data formats while searching for specific genomic regions. A comparison of our non-relational and generic solution, with an existing relational approach (developed for tabular data types using 2 bits to store genotypes), showed reduced importing time to handle 50M SNPs (PLINK format) achieved by the relational schema. Our experimental results also reinforce that data conversion is a costly step required to manage genomic data into both relational and non-relational database systems, and therefore, must be carefully treated for large applications. 相似文献
85.
Berti C Roncoroni L Falini ML Caramanico R Dolfini E Bardella MT Elli L Terrani C Forlani F 《Journal of agricultural and food chemistry》2007,55(6):2482-2488
The effects of chemical (acid-heating treatment) and enzymatic (microbial transglutaminase, TGase) modification (deamidation) of gluten proteins on their physicochemical and celiac disease-related properties were studied. Ammonia release, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and sample solubility analyses were employed to check the extent of gluten modification. Among different treatments achieved, the acid-heating treatment performed at 90 degrees C for 3 h induced gluten deamidation, paralleling an increase of gluten solubility without relevant proteolysis. Changes in the immunoreactivity of celiac IgA anti-gliadin antibodies (AGAs) to modified gluten proteins were detected by using a competitive indirect enzyme-linked immunosorbent assay method. Chemical deamidation by acid-heating treatment of gluten lowered IgA-AGA immunoreactivity. IgA-AGA immunoreactivity to gliadins was increased when they were submitted to TGase-catalyzed deamidation. The acid-heating treatment of gluten reduced its cytotoxic activity on human colon adenocarcinoma LoVo cell line. These results showed that chemical deamidation of gluten may be envisaged as a way to lower the potential risk for celiac people due to widespread use of gluten as a food additive. 相似文献
86.
Rosa RM Melecchi MI da Costa Halmenschlager R Abad FC Simoni CR Caramão EB Henriques JA Saffi J de Paula Ramos AL 《Journal of agricultural and food chemistry》2006,54(19):7324-7330
The genus Hibiscus thrives in a variety of climates and produces a diversity of natural compounds with bioactive properties. We have studied the chemical composition and the in vivo antioxidant properties of Hibiscus tiliaceus L. methanolic flower extract, as well as its mutagenic/antimutagenic effects. Vitamin E and some stigmasterol derivatives that might confer an antioxidant effect to the extract were present. Treatment with this extract protected several Saccharomyces cerevisiae strains defective in antioxidant defenses against H2O2 and t-BOOH cytotoxicities, showing a clear antioxidant activity. The effect is the same for all strains used, independent of the antioxidant defense disrupted, suggesting that protection may be due to molecules that act as versatile and wide spectrum nonenzymatic antioxidants, such as vitamins or phytosterols. The extract was not mutagenic in either Salmonella typhimurium or S. cerevisiae and showed a significant antimutagenic action against oxidative mutagens in S. cerevisiae. 相似文献
87.
Campos E Moraes J Façanha AR Moreira E Valle D Abreu L Manso PP Nascimento A Pelajo-Machado M Lenzi H Masuda A Vaz Ida S Logullo C 《Veterinary parasitology》2006,138(3-4):349-357
The present work evaluates the kinetics of utilization of the main potential energy sources throughout the embryonic developmental stages of Boophilus microplus. The embryonic development of this arthropod is completed in 21 days. Cellularization of the blastoderm occurs on the 6th day and is rapidly followed by germ band extension and segmentation, whose first signs are visible on the 7th day. Cellularization is typically a maternal-driven process, carried out by molecular determinants deposited in the oocyte during oogenesis. On the other hand, segmentation is of zygotic nature, being the consequence of the synthesis of various components by the growing embryo. The enhancement in total B. microplus RNA was observed after cellularization, corroborating the replacement of maternal-driven processes by embryonic zygotic expression. An abrupt increase in oxygen consumption was observed from cellularization until the 8th day of development. The reduction in dry weight at the same period and the susceptibility of oxygen consumption to KCN suggest that the respiration process is activated during early embryonic development. A marked decrease in total lipid content occurred between the 5th and 7th days of development, suggesting this is the main energy source for cellularization. A major reduction in carbohydrate content occurred later, between the 7th and 9th days, and it could be assigned to the morphological segmentation of the embryo. Although the total amount of proteins remains unchanged from oviposition to hatching, a 15% reduction in vitellin (VT) content was observed before cellularization, up to the 4th day after egglaying. This observation was correlated to the synthesis of new proteins needed to support early embryo development. Additional 20% of VT was consumed thereafter, mainly at the end of embryogenesis, and in this case VT is probably used as energy source to the older embryo. Altogether, these data indicate different energy sources for maternal and zygotic driven processes. 相似文献
88.
A. G. C. Signoretti M. F. G. V. Peñaflor L. S. D. Moreira N. C. Noronha J. M. S. Bento 《Journal of pest science》2012,85(1):101-107
Herbivore-attacked plants produce specific volatile substances that represent important cues for host finding by natural enemies. The fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is a voracious herbivore and usually feed on maize in all periods of the day. Given that plant needs light to synthesize de novo herbivore-induced volatiles, volatile blend may be changed depending on time of the day the plant is induced, what could interfere in natural enemy foraging. In this sense, the current study aimed to investigate differential attractiveness of maize elicited by fall armyworm regurgitant under light and dark conditions to its specialist larval parasitoid Campoletis flavicincta (Ashmead) (Hymenoptera: Ichneumonidae). All bioassays were conducted in Y-tube olfactometer to assess parasitoid response to odors from undamaged maize, mechanical damage, and regurgitant-treated plants at 0–1, 5–6, and 24–25 h after induction. The results showed that naïve wasps were attracted to volatiles emitted by nocturnal regurgitant-treated maize at 5–6 h, but not to odors from diurnal regurgitant-treated plants. The differential attractiveness is likely due to blend composition as nocturnal regurgitant-treated plants emit aromatic compounds and the homoterpene (3E)-4,8-dimethyl-1,3,7-nonatriene in larger amounts than diurnal-treated plants. 相似文献
89.
Leandro Maia MV MS Maria Vernica de Souza MV MS DS Pharmacist-Biochemical Jos Ivo Ribeiro Júnior BS MS DS Acio Carlos de Oliveira Geraldo Eleno Silveira Alves MV MS DS Larcio dos Anjos Benjamin MV MS DS Yamê Fabres Robaina Sancler Silva Bruna Mota Zandim MV Jos do Carmo Lopes Moreira MD MS 《Journal of Equine Veterinary Science》2009,29(8):618-626
This study was carried out to evaluate the effect of platelet-rich plasma (PRP) on the treatment of tendinopathy induced in the superficial digital flexor tendon (TFDS) of horses, by using histologic evaluation. Six healthy crossbred geldings aged 8 to 15 years (12 ± 3) were used. The TFDS tendinopathy was provoked in both forelimbs, by intratendinous administration of 2.5 mg collagenase (2.5 mg/mL), and this procedure was considered as the beginning of the experimental phase. At 12 days after induction of the tendinopathy, the animals were subjected to the following treatments: (1) in the lesion caused in the right superficial digital flexor tendon (PRP-treated group), 2.5 mL PRP activated with calcium chloride at 0.0125 mol/L at concentrations from 320,000 to 500,000 platelets/μL, were injected; (2) in the tendinopathy of the left SDFT (control group), 2.5 mL 0.9% saline solution was administrated. Thirty-six days after the treatments, a biopsy of the injured area was performed for histologic evaluation. In both groups, the histologic analysis showed an increase in the fibroblastic density, as well as the presence of neovascularization, lymphocytes, and plasmocytes infiltrate and tissue organization at variable intensity. In the PRP-treated group, the SDFT was more organized, with the collagen fibers and fibroblasts being better arranged on the tendon matrix. The numbers of the fibroblasts and blood vessels did not differ between the groups. Histologic evaluation 36 days after tendinopathy showed that injuries under a single PRP treatment present a more uniform and organized tissue repair when compared with the control group. 相似文献
90.
Alexandre R. Cabral Marlon A. Capanema Julia Gebert Joao F. Moreira Louis B. Jugnia 《Water, air, and soil pollution》2010,209(1-4):157-172
Stable isotope analyses were performed on gas samples collected within two instrumented biocovers, with the goal of evaluating CH4 oxidation efficiencies (f 0). In each of the biocovers, gas probes were installed at four locations and at several depths. One of the biocovers was fed with biogas directly from the waste mass, whereas the other was fed through a gas distribution system that allowed monitoring of biogas fluxes. While the f 0 values obtained at a depth of 0.1 m were low (between 0.0% and 25.2%) for profiles with poor aeration, they were high for profiles with better aeration, reaching 89.7%. Several interrelated factors affecting aeration seem to be influencing f 0, including the degree of water saturation, the magnitude of the biogas flux and the temperature within the substrate. Low f 0 values do not mean necessarily that little CH4 was oxidized. In fact, in certain cases where the CH4 loading was high, the absolute amount of CH4 oxidized was quite high and comparable to the rate of CH4 oxidation for cases with low CH4 loading and high f 0. For the experimental biocover for which the CH4 loading was known, the oxidation efficiency obtained using stable isotopes (f 0?=?55.67% for samples taken inside flux chambers) was compared to the value obtained by mass balance (f 0?=?70.0%). Several factors can explain this discrepancy, including the high sensitivity of f 0 to slight changes in the isotopic fractionation factor for bacterial oxidation, α ox, uncertainties related to mass flow metre readings and to the static chamber method. 相似文献